. Author manuscript; available in PMC: 2020 Aug 1.

Published in final edited form as: Neurogastroenterol Motil. 2019 May 29;31(8):1–11. doi: 10.1111/nmo.13652

Table 1.

Tecnical information regarding the specific protein detection via WB tecnique.

Protein		MW (KDa)	Total protein amount (μg)	Acrylamide concentration	Antibody concentration	Reference protein
OCLN		60	20	12%	2 μg/ml	GAPDH
OCLN assembly	Oligomers	>150	40	4 – 20%
	Dimers	110–120
	Monomers	53–65
	LMW	37–35
CLDN4		22	100	15%	2 μg/ml	GAPDH
ZO-1		195	20	5%	1:500	Vinculin
VIP		19	100	15%	1:200	GAPDH
GFAP		50	100	15%	1:3000	GAPDH
GAPDH		36	20; 40; 100	12; 15; 4–20%	1:1000	-
Vinculin		129	20	5%	1:1000	-