Skip to main content
. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Alcohol. 2019 Jan 18;79:93–103. doi: 10.1016/j.alcohol.2019.01.006

Figure 1. Schema of experimental timeline.

Figure 1.

(A) Pair-housed Long-Evans rats were exposed to five cycles of intermittent ethanol vapor inhalation between PD 28-44 and tested in adulthood for two-bottle choice (2BC) and quinine adulterated ethanol drinking, operant ethanol self-administration, and sucrose preference. Litter mate control rats were exposed to plain air during the ethanol exposure period. (B) Intermittent 2BC drinking consisted of 20 cumulative weeks of home cage ethanol drinking. To examine aversion resistant drinking, the effects of quinine adulteration of the ethanol solution was examined after two 7-week periods of intermittent 2BC drinking separated by the first quinine test. Both quinine test sessions involved adulteration of the ethanol bottle with increasing concentrations of quinine. Each concentration of quinine was tested on Wednesdays of each of the three weeks, with unadulterated ethanol provided on the Monday and Friday of each week of quinine testing.