Figure 2.

Helicobacter pylori infection promotes proliferation and inflammation and induces YAP1 overexpression and nuclear translocation. (A, B, C) PBS and hp26695 were added to AGS, BGC‐823, and GES‐1 cells, respectively (MOI = 100:1). The cells were harvested at 2, 4, 6, and 8 h, and Western blot analysis was performed. YAP1 and IL‐1β expression increased, while phosphorylation at the S127A position on YAP1 was decreased in a time‐dependent manner. (D, E) Immunofluorescence staining of BGC‐823 cells infected with CagA‐positive and CagA‐negative Hp strains, respectively (****=P < 0.0001, unpaired t test). (F) YAP1 was upregulated in AGS cells overexpressing CagA. (G) Colony formation of AGS and BGC cells. PBS or hp26695 was added to cells 72 h after knocking down YAP1. Quantification is shown at the bottom (data are presented as the mean ± SD of three independent experiments, *=P < 0.05, **=P < 0.01, ***=P < 0.001, unpaired t test)