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. 2018 Dec 3;51(3):1167–1179. doi: 10.4143/crt.2018.526

Fig. 3.

Fig. 3.

The synergistic effect was observed with combinations of AZD6738 and cytotoxic chemotherapeutic agents. (A) Anti-proliferative effects of combination chemotherapy of AZD6738 and cytotoxic agents (cisplatin, gemcitabine, and 5-fluorouracil [5-FU]) evaluated by MTT assays. The combination indexes for the combinations of AZD6738 and the cytotoxic agents at the ED50 were calculated by the Chou and Talalay method in the SNU478, SNU869, SNU245, and SNU2670 cell lines (combination index [CI] > 1, antagonistic effect; CI=1, additive effect; CI < 1, synergistic effect). (B) Colony-forming assays were performed to demonstrate the combined effect of AZD6738 and cisplatin. The concentration of each drug is indicated in the graph. The data represent mean±SE of three independent experiments. **p < 0.01. (C) Western blot analyses were performed to evaluate the anti-proliferative effect of AZD6738 and cisplatin on biliary tract cancer cell lines. PARP, poly(ADP-ribose) polymerase.