FIG 3.

Growth kinetics of rHAZV versus parental isolate. (A) Titers of infectious rHAZV and its parental strain of HAZV harvested at 24-h intervals for 4 days following infection of SW13 cells at an MOI of 0.001. (B) Representative plaque assays used to determine titers of infectious HAZV and rHAZV in panel A, showing plaque morphology to be similar in the two instances. Serial dilutions were generated from virus harvested 24 h postinfection and used to infect SW13 monolayers for 1 h prior to addition of a methylcellulose overlay. Cells were then fixed and stained at 6 days postinfection, and viral plaques were counted.