FIG 5.

Impact of CD4⁺ and CD8⁺ T cell depletion in the lungs as assessed by flow cytometry. BALB/c mice (7 weeks old; n, 5 per group) were immunized i.m. at day 0 and day 21 with either buffer alone, different doses of post-F antigen with GLA-SE (2.5 μg GLA in 2% SE) as an adjuvant, or FI-RSV. A negative-control group was immunized with a nonrelevant antigen (CMV gB) with GLA-SE as an adjuvant. Naïve animals were left untreated. At days 29, 32, and 35, groups were treated i.p. with 250 μg of anti-CD4 (A) or anti-CD8 (B) antibodies as indicated. Four days after i.n. challenge with 10⁶ PFU of wild-type (wt) RSV A2, animals were sacrificed. Lungs were harvested, and cells were isolated and surface stained as described in Materials and Methods. Individual results and medians with ranges are presented.