FIG 1.

trans-Complementation of movement-defective ToMV and PVX. (A and B) Leaves of N. benthamiana plants were agroinfiltrated with bacterial mixtures carrying transcription plasmid for ToMV-GFPΔMP (A) or PVX-GFPΔp25 (B), plus a binary plasmid (pGD-X) to express SYNV sc4, PYDV Y, RYSV P3, RSMV P3, TYMaV P3, ToMV MP, PVX p25, or GUS as a negative control, as indicated on the top of each panel. Images were taken with a fluorescence microscope at 60 hpi (A) or 8 dpi (B). Scale bars = 200 μm. Note that the bars in the ToMV MP and PVX p25 images in panel B are half the lengths of the bars in other panels due to more extensive movement with these treatments.