TABLE 1.
trans-Complementation of cell-to-cell movement of ToMV-GFPΔMP and PVX-GFPΔMP
| MP | No. of foci by no. of cells in clustera
|
Total no. of foci counted |
Movement efficiency (≥ 4 cells) (%) |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
2–3 |
4–5 |
6–7 |
8–9 |
≥10 |
|||||||||||
| ToMV | PVX | ToMV | PVX | ToMV | PVX | ToMV | PVX | ToMV | PVX | ToMV | PVX | ToMV | PVX | ToMV | PVX | |
| GUS | 55 | 53 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 60 | 60 | 0 (0) | 0 (0) |
| SYNV sc4 | 13 | 32 | 21 | 14 | 13 | 11 | 8 | 3 | 5 | 0 | 0 | 0 | 60 | 60 | 26 (43.3) | 14 (23.3) |
| PYDV Y | 17 | 29 | 17 | 11 | 10 | 9 | 6 | 5 | 7 | 4 | 3 | 2 | 60 | 60 | 26 (43.3) | 20 (33.3) |
| RYSV P3 | 14 | 29 | 18 | 14 | 10 | 7 | 7 | 6 | 5 | 2 | 6 | 2 | 60 | 60 | 28 (46.7) | 17 (28.3) |
| RSMV P3 | 14 | 22 | 17 | 10 | 5 | 7 | 10 | 5 | 6 | 6 | 8 | 10 | 60 | 60 | 29 (48.3) | 28 (46.7) |
| TYMaV P3 | 12 | 25 | 12 | 13 | 9 | 8 | 10 | 3 | 6 | 5 | 11 | 6 | 60 | 60 | 36 (60.0) | 22 (36.7) |
| PVX P25 | 53 | 2 | 7 | 1 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 11 | 60 | 20 | 0 (0) | 17 (85.0) |
| ToMV MP | 4 | 3 | 10 | 2 | 7 | 1 | 6 | 0 | 11 | 4 | 22 | 10 | 60 | 20 | 46 (76.7) | 15 (75.0) |
a
ToMV, ToMV-GFPΔMP infection foci at 60 h postinfiltration. PVX, PVX-GFPΔMP infection foci at 8 days postinfiltration.