FIG 1.

Effects of immunoproteasome deficiency on MAV-1 replication in lungs of adult mice. Adult B6 and TKO mice were infected intranasally with MAV-1. (A) qPCR was used to quantify MAV-1 genome copies in the lungs. DNA viral loads are expressed as copies of MAV-1 genome per 100 ng of input DNA. (B) RT-qPCR was used to quantify MAV-1 TPL mRNA levels in the lungs. mRNA levels are expressed in arbitrary units standardized to GAPDH. Individual circles represent values for individual mice (n = 7 to 13 per group), and horizontal bars represent geometric means (A) or means (B) for each group. Statistical comparisons were made using two-way ANOVA, followed by Bonferroni’s multiple-comparison tests. No statistically significant differences were identified.