FIG 4.

Effects of immunoproteasome deficiency on survival and MAV-1 replication in neonatal mice. B6 and TKO mice were infected intranasally with MAV-1 at 7 days of age. (A) Survival was monitored throughout the course of the experiments (n = 9 B6 mice, n = 19 TKO mice). Statistical comparisons were made using the log-rank (Mantel-Cox) test. (B to G) Lungs, hearts, and livers were harvested from mice at 7 and 9 dpi (n = 8-15 per group). (B to D) qPCR was used to quantify MAV-1 genome copies in lungs, hearts, and livers. DNA viral loads are expressed as copies of MAV 1 genome per 100 ng of input DNA. (E to G) RT-qPCR was used to quantify MAV-1 TPL mRNA levels in lungs, hearts, and livers. The data are shown in arbitrary units standardized to GAPDH. In panels B to G, individual circles represent values for individual mice, and horizontal bars represent geometric means (B to D) or means (E to G) for each group. Statistical comparisons were made using two-way ANOVA, followed by Bonferroni’s multiple-comparison tests. No statistically significant differences between B6 and TKO mice were identified at either time point.