FIG 5.

Effects of immunoproteasome deficiency on virus-induced inflammation in neonatal mice. B6 and TKO mice were infected intranasally with MAV-1 at 7 days of age. Control animals were mock infected with conditioned media. Lungs, hearts, and livers were harvested from mice at 7 and 9 dpi. RT-qPCR was used to quantify mRNA levels of the indicated cytokines in lungs (A to C), hearts (D to F), and livers (G to I). mRNA levels are expressed in arbitrary units standardized to GAPDH. Individual circles represent values for individual mice (n = 8 to 16 mice per group), and horizontal bars represent means for each group. Statistical comparisons were made using two-way ANOVA, followed by Bonferroni’s multiple-comparison tests. *, P < 0.05; **, P < 0.01, and ***, P < 0.001, comparing mock-infected and infected mice of the same genotype. ††, P < 0.01, and †††, P < 0.001, comparing infected B6 and TKO mice. (J) Hematoxylin-and-eosin-stained sections were prepared from paraffin-embedded lungs, hearts, and livers. Scale bars, 100 μm.