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. 2019 Jul 17;93(15):e00569-19. doi: 10.1128/JVI.00569-19

FIG 7.

FIG 7

Age-based increases in immunoproteasome subunit expression in lungs of uninfected mice. RT-qPCR was used to quantify mRNA levels of the indicated immunoproteasome subunits (A to C) and IFN-γ (E) in lungs harvested from uninfected B6 mice at the indicated ages (n = 3 to 8 mice per group). (D) Immunoproteasome subunit protein was detected by immunoblot in whole lung homogenates from B6 mice at the indicated ages (n = 4 per group). (F to H) RT-qPCR was used to quantify mRNA levels of the indicated immunoproteasome subunits in lungs harvested from uninfected adult (36 to 37 days old) B6 and IFN-γ−/− mice (n = 6 per group). mRNA levels are expressed in arbitrary units standardized to GAPDH. Individual circles represent values for individual mice, and horizontal bars represent means for each group. Statistical comparisons in panels A to C and panel E were made using the Kruskal-Wallis test, followed by Dunn’s multiple-comparison tests. Statistical comparisons in panels F to H were made using Mann-Whitney test (*, P < 0.05; **, P < 0.01).