. 2019 Jul 17;93(15):e00305-19. doi: 10.1128/JVI.00305-19

TABLE 2.

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort (n = 399)^a

Explanatory variable	Peak IL-2⁺ SFC		Peak IFN-γ⁺ SFC		Peak IFN-γ⁺ IL-2⁺ SFC
Explanatory variable	RCE	P	RCE	P	RCE	P
Age	0.03	0.96	−0.41	0.69	−0.19	0.7
Baseline responses	1.01	<0.0001	1.46	<0.0001	1.08	<0.0001
CD4⁺ IL-10⁺	−15.1	0.32	−6.64	0.78	−7.14	0.52
CD8⁺ IL-10⁺	10.03	0.43	−2.43	0.90	−0.06	0.99
CD4⁺ FOXP3⁺ CD25⁺	82.71	0.15	193.7	0.03	56.17	0.19
CD8⁺ FOXP3⁺ CD25⁺	−60.1	0.17	−159	0.02	−37.5	0.24
CD4⁺ FOXP3⁺	−36.8	<0.01	−35.7	0.09	−20.7	0.04
CD8⁺ FOXP3⁺	26.77	0.12	27.56	0.30	13.86	0.28
CD4⁺ TGF-β⁺	19.06	0.09	9.95	0.56	6.16	0.46
CD8⁺ TGF-β⁺	−12.7	0.08	1.89	0.86	−3.67	0.49
CD4⁺ CD28⁻ CD57⁺	−1.84	0.09	−3.03	0.07	−1.03	0.19
CD8⁺ CD28⁻ CD57⁺	−0.31	0.54	0.27	0.73	−0.15	0.67
CD4⁺ PD1⁺	8.87	0.16	2.99	0.76	2.74	0.56
CD8⁺ PD1⁺	−19.9	<0.01	−1.34	0.87	−11.4	<0.01
CD4⁺ CD31⁺	−18.3	0.09	27.94	0.09	−5.73	0.47
CD8⁺ CD31⁺	13.21	0.21	−29.1	0.07	9.78	0.21

Boldface type indicates statistically significant effects defined by a P value of <0.02 to adjust for multiple comparisons. Abbreviations: VZV, varicella-zoster virus; Th1, T helper 1; CTL, cytotoxic T lymphocytes; RCE, regression coefficient estimate (indicates the magnitude of the effect of the explanatory variable on the outcome measure); SFC, spot-forming cells/10⁶ PBMC; baseline responses, prevaccination responses in the same category as the outcome measure (e.g., prevaccination IFN-γ SFC as an explanatory variable for peak IFN-γ SFC or prevaccination IL-2 SFC as an explanatory variable for peak IL-2 SFC, etc.).