. 2019 Jul 17;93(15):e00305-19. doi: 10.1128/JVI.00305-19

TABLE 3.

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort (n = 95)^a

Explanatory variable	Peak IL-2⁺ SFC		Peak IFN-γ⁺ SFC		Peak IFN-γ⁺ IL-2⁺ SFC
Explanatory variable	RCE	P	RCE	P	RCE	P
Age	1.75	0.14	−0.69	0.67	1.20	0.15
Baseline responses	1.18	<0.0001	1.64	<0.0001	1.21	<0.0001
VZV-specific CD4⁺ CD39⁺	−76.5	0.02	−71.7	0.09	−51.2	0.03
VZV-specific CD8⁺ CD39⁺	18.3	0.32	4.22	0.86	23.9	0.07
VZV-specific CD4⁺ FOXP3⁺	107.4	0.04	141.6	0.04	86.5	0.021
VZV-specific CD8⁺ FOXP3⁺	1.18	0.96	48.2	0.17	10.8	0.57
VZV-specific CD4⁺ IL-10⁺	−23.5	0.68	−122	0.1	9.6	0.81
VZV-specific CD8⁺ IL-10⁺	30.3	0.41	73.8	0.12	0.46	0.99
VZV-specific CD4⁺ TGF-β⁺	−17.9	0.46	9.9	0.75	−6.0	0.73
VZV-specific CD8⁺ TGF-β⁺	1.3	0.92	−2.1	0.91	−1.8	0.85

Boldface type indicates statistically significant effects defined by a P value of <0.02 to adjust for multiple comparisons. Abbreviations: VZV, varicella-zoster virus; Th1, T helper 1; CTL, cytotoxic T lymphocytes; RCE, regression coefficient estimate (indicates the magnitude of the effect of the explanatory variable on the outcome measure); SFC, spot-forming cells/10⁶ PBMC; baseline responses, prevaccination responses in the same category as the outcome measure (e.g., prevaccination IFN-γ SFC as an explanatory variable for peak IFN-γ SFC or prevaccination IL-2 SFC as an explanatory variable for peak IL-2 SFC, etc.).