FIG 4.

Characterization of biophysical properties of ΔC-CHIKV virions. (A) Plaque morphology of WT CHIKV and ΔC-CHIKV on BHK-21 cells. (B) Growth kinetics of WT CHIKV and ΔC-CHIKV. BHK-21 cells were infected with WT CHIKV or ΔC-CHIKV mutants at an MOI of 0.01. Viral titers were measured by a plaque assay on BHK-21 cells. (C) Neutralization assay using mouse sera against WT CHIKV conducted with a PRNT. (D) Thermal stability of WT and ΔC-CHIKV. The corresponding eGFP reporter viruses, WT CHIKV-eGFP and ΔC-CHIKV-eGFP, were used as described in Materials and Methods. Shown are mean half-life values obtained from three independent experiments performed in triplicate. The bars indicate the standard deviations from three independent experiments.