FIG 6.
Analysis of the composition of purified ΔC-CHIKV virions. (A) SDS-PAGE analysis followed by Coomassie staining for fraction 8 of WT CHIKV and fraction 4 of ΔC-CHIKV by sucrose density gradient centrifugation. Gels were loaded with purified ΔC-CHIKV at approximately 3 × 104 PFU or an equivalent volume of purified WT CHIKV at approximately 5 × 106 PFU. (B) Western blot assays to analyze viral nsPs with antiserum specifically targeting CHIKV nsP1 to nsP4 and exosomes with marker CD63 antibody of purified ΔC-CHIKV and WT CHIKV virions. Gels were loaded with purified ΔC-CHIKV or WT CHIKV as described above for panel A, and cell lysates for the detection of ΔC-CHIKV or WT CHIKV or mock-infected cells were adjusted for similar signals. Three independent experiments were performed, with similar results, and one of the representative data sets is presented.