FIG 3.

Quantification of SIV DNA and RNA from CD4⁺ T cells and CD11b⁺ cells isolated from tissues. CD4⁺ T cells (A and B) and CD11b⁺ cells (C, D, and E) were isolated from spleen, lung, and PBMCs from seven SIV-infected ART-suppressed macaques and two SIV-infected untreated macaques. Cellular DNA and RNA were then extracted and analyzed for SIV gag DNA (left), SIV gag RNA (middle), and SIV tat/rev RNA (right) by PCR. The limit of quantitation (LOQ) for ddPCR is 1 copy per reaction, and that for qPCR is 10 copies per reaction. The dashed lines represent the LOQ for ddPCR. Open symbols indicate animals that were treated with LRAs in vivo; closed symbols indicate animals that were not treated with LRAs.