The macrophage population in culture is stable over time. IFN-β gene (A, C, and E) and SIV gag (B, D, and F) DNA copies were quantitated by qPCR in CD11b+ cells isolated from spleen, lung, and PBMCs before being plated for Mϕ QVOA and also from cells in the QVOA wells at 14 or 21 days postplating. Values are presented as the number of copies per 106 cells. Open symbols indicate animals that were treated with LRAs in vivo; closed symbols indicate animals that were not treated with LRAs. D0, D14, and D21, days 0, 14, and 21, respectively.