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Supernatants from Mϕ QVOA cultures contain viruses that establish de novo infection in activated macaque PBMCs. Activated PBMCs from a healthy pigtailed macaque were spinoculated with culture supernatant from positive Mϕ QVOA wells with spleen tissue (A), lung tissue (B), and blood (C) from the seven suppressed macaques. The graphs show the viral kinetics over time postinfection, as measured by an SIV RNA RT-qPCR. Each line represents viral replication from one Mϕ QVOA well.
