TABLE 2.
Quantitation of IUPM in CD4+ T cell and monocyte/macrophage QVOA
| Animal code | IUPMa
|
||||
|---|---|---|---|---|---|
| CD4+ T cell QVOA |
Monocyte/macrophage QVOA |
||||
| Spleen | Blood | Spleen | Lung | Blood | |
| Pm11 | 1.27 | 0.84 | 0.07 | 0.06 | 0.06 |
| Pm12 | <LOD | <LOD | 0.79 | 0.29 | 0.40 |
| Pm21 | 0.26 | 0.29 | 0.47 | 0.06 | 0.22 |
| Pm22 | 0.16 | 0.37 | 0.66 | 0.06 | 1.00 |
| Pm23 | 1.86 | 0.16 | 0.06 | 0.22 | 3.44 |
| Pm24 | 0.16 | 0.46 | 0.36 | 0.42 | 0.63 |
| Pm25 | 0.06 | 0.06 | 0.40 | 0.08 | 0.39 |
a
IUPM was quantitated as described by Avalos et al. (33). IUPM values represent the presence or absence of positive wells with more than 50 SIV gag RNA copies/ml of supernatant as determined by RT-qPCR, as described in the legend to Fig. 5. Values reflect maximum likelihood estimates of the infection frequency (in numbers of IUPM). Given the resolution of the assay, the 95% confidence interval was typically 0.2 to 4 times the reported value. <LOD, less than the limit of detection.