TABLE 3.
Frequency of infected CD4+ T cells among macrophages isolated from spleen, lung, and blood Mϕ QVOAs
| Compartment and animal code | No. of CD4+ T cell IUPMa | No. of CD3+ T cells in Mϕ QVOA well | % CD3+ CD4+ CD8− T cells in blood at necropsy | No. of CD4+ T cells in Mϕ QVOA well | Probability of an infected CD4+ T cell present in Mϕ QVOA well | % chance an infected CD4+ T cell is present in an Mϕ QVOA well | IUPM Mϕ QVOA |
|---|---|---|---|---|---|---|---|
| Spleen | |||||||
| Pm11 | 1.27 | 0.88 | 50.9 | 0.45 | 5.69E−07 | 0.00 | 0.07 |
| Pm12 | 0.00 | 0.53 | 60.2 | 0.32 | 0.00 | 0.00 | 0.79 |
| Pm21 | 0.26 | 0.56 | 55.2 | 0.31 | 0.00 | 0.00 | 0.47 |
| Pm22 | 0.16 | 0.46 | 56.8 | 0.26 | 0.00 | 0.00 | 0.66 |
| Pm23 | 1.86 | 0.12 | 56.4 | 0.07 | 0.00 | 0.00 | 0.06 |
| Pm24 | 0.16 | 0.52 | 45.8 | 0.24 | 0.00 | 0.00 | 0.36 |
| Pm25 | 0.06 | 0.54 | 48.9 | 0.26 | 0.00 | 0.00 | 0.40 |
| Lunga | |||||||
| Pm11 | 0.84 | 0.37 | 50.9 | 0.19 | 0.00 | 0.00 | 0.06 |
| Pm12 | 0.00 | 0.40 | 60.2 | 0.24 | 0.00 | 0.29 | |
| Pm21 | 0.29 | 2.40 | 55.2 | 1.32 | 0.00 | 0.00 | 0.06 |
| Pm22 | 0.37 | 0.36 | 56.8 | 0.20 | 0.00 | 0.00 | 0.06 |
| Pm23 | 0.16 | 0.46 | 56.4 | 0.26 | 0.00 | 0.00 | 0.22 |
| Pm24 | 0.46 | 0.58 | 45.8 | 0.27 | 0.00 | 0.00 | 0.42 |
| Pm25 | 0.06 | 0.28 | 48.9 | 0.14 | 0.00 | 0.00 | 0.08 |
| PBMC | |||||||
| Pm11 | 0.84 | 0.49 | 50.9 | 0.25 | 0.00 | 0.00 | 0.06 |
| Pm12 | 0.00 | 0.62 | 60.2 | 0.37 | 0.00 | 0.40 | |
| Pm21 | 0.29 | 0.64 | 55.2 | 0.35 | 0.00 | 0.00 | 0.22 |
| Pm22 | 0.37 | 0.19 | 56.8 | 0.11 | 0.00 | 0.00 | 1.00 |
| Pm23 | 0.16 | 0.15 | 56.4 | 0.09 | 0.00 | 0.00 | 3.44 |
| Pm24 | 0.46 | 0.36 | 45.8 | 0.16 | 0.00 | 0.00 | 0.63 |
| Pm25 | 0.06 | 0.15 | 48.9 | 0.07 | 0.00 | 0.00 | 0.39 |
a
CD4+ T cell IUPM values were based on blood QVOA results.