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. 2019 Jul 18;9:10447. doi: 10.1038/s41598-019-46909-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient elastase perfusion induces AAA. Mice were transiently perfused with elastase on day 0 and increase in aortic diameter (AD) was measured immediately post-perfusion, and on day 7 or day 14. Elastase perfusion resulted in ∼70% increase in AD on day 0 (post perfusion). AAA was defined as an increase in AD of greater than 100% on day 14 compared with the AD measured prior to elastase perfusion. AAA is expressed as the increase in AD in mm (A) or % (B). AAA was accompanied by elastic fiber degradation on day 14 (C). Scale bar = 250 um.