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. 2019 Jul 18;9:10425. doi: 10.1038/s41598-019-46929-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolic changes related to glucose metabolism. (a) Metabolite changes in the skeletal muscle of young and aged mice are shown. Relative metabolite changes shown in the graphs were obtained by CE-TOFMS (Supplementary Table 1). Open bars, young mice; filled bars, aged mice. Data are expressed as mean ± SD (N = 5); **p < 0.01, *p < 0.05. Gene expression of (b) glucose metabolism and (c) myosin heavy chain in skeletal muscle from young and aged mice. Open bars, young mice; filled bars, aged mice. Data are expressed as mean ± SE (N = 5); ***p < 0.001, **p < 0.01. (d) Feret’s diameter of total fiber in tibilias anterior (TA) muscle. (e) Immunohistochemical analysis of the fiber-type composition in TA muscles. Red, type IIa and laminin; unstained, type IIx; green, type IIb. Scale bars 100 μm. (f) Myofiber Feret’s diameter of individual fiber types in TA muscle. ***p < 0.001. There were few type I fibers in all samples.