Fig. 2. NBT exhibits potent antitumor activity in a metastasis mouse model.

Six-week-old female nude mice were injected with 1 × 10⁶ MDA-MB-231 cells in the tail vein. After injection, the mice were divided into three groups (n = 8) and administered DMSO (vehicle group), NBT (2.5 mg/kg per days), or 5-FU (20 mg/kg per 2 days) using intraperitoneal injection. a Representative lung images taken from mice at 2 months. b Quantitative analysis of metastatic nodes in the lung. c Representative HE-staining of lung tissues from the three groups. d Lung weight analysis after treatment. e Body weight analysis every other day throughout the entire experiments. *P < 0.05, **P < 0.01, ***P < 0.001