Fig. 5. Increased proteasome activity in the P23H retina decreases photoreceptor cell death.

a Representative TUNEL staining images and b quantification of TUNEL-positive photoreceptors from retinas of C57 (wild-type) mice or P23H mice treated with rolipram or vehicle-only as control (CTL) for 2 weeks. Scale bar: 50 µm. Representative western blots from retinas of C57 mice or P23H mice treated with rolipram or vehicle-only probed in c for pMLKL, total MLKL or GAPDH (loading control), with quantification of protein levels, or in d for pRIPK3, RIPK3 and GAPDH, with levels of pMLKL and pRIPK3 quantified by normalizing to levels of total MLKL and RIPK3 respectively. Results are shown as individual symbols representing data values along with mean and standard deviation (SD). Statistical analyses with one-way ANOVA. *p ≤ 0.05. **p ≤ 0.01. ***p ≤ 0.001. ****p ≤ 0.0001