Fig. 2.

tyra-2 is required for osas#9 mediated aversive responses independent of tyramine. a Screen for receptors required to mediate osas#9 avoidance. tyra-2 lof animals are defective in osas#9 avoidance response. n ≥ 4 trials. b Two alleles of tyra-2 lof animals (tm1846 and tm1815), are defective in osas#9 avoidance behavior. n ≥ 4 trials. c wild type, wt, and tyra-2 lof mutants showed no significant differences when subjected to known chemical deterrents. n ≥ 3 trials. d Wild type animals avoided osas#9 at 1 and 10 µM, while both tyra-2 lof mutants avoid at only 100 µM. n ≥ 8 trials. e Expression of tyra-2 receptor is dependent on the physiological state of the animal. RT-qPCR analysis starved animals indicates a nearly twofold upregulation of tyra-2. Data are displayed as the ratio of endogenous tyra-2 mRNA to ama-1 mRNA from three independent RT-qPCR experiments. n = 3 trials. f osas#9 avoidance response is not dependent on endogenous tyramine. Two different alleles of tdc-1 lof animals (n3419 and n3420) which are deficient in tyramine biosynthesis, exhibit normal response to osas#9. n ≥ 7 trials. Data presented as mean ± S.E.M; *p < 0.05, **p < 0.01, ***p < 0.001, one factor ANOVA with Sidak’s multiple comparison posttest except for 2D and 2E, where Student’s t-test was used