Fig. 4.

tyra-2 expression in sensory neurons is required for sensitivity to osas#9. a A transcriptional rescue construct, pnhr-79::tyra-2::RFP, exhibits expression of tyra-2 in both ASH and ADL neurons (×40 magnification, scale bar denotes 10 µm). b Rescue of tyra-2 in ASH/ADL neurons fully reconstituted behavioral response to 1 µM osas#9. n ≥ 4 trials. c Rescue of tyra-2 in ASH neurons in a tyra-2 lof (blue) background show no difference compared to calcium transients of wild type animals expressing GCaMP3 in ASH neurons (black) when exposed to 1 µM osas#9 (gray-shaded region), while tyra-2 lof animals show a clear loss of calcium transcients in ASH (red), n ≥ 10 animals. d Maximum fluorescence intensity in transgenic worms before (solvent control) and during exposure to 1 µM osas#9. e Sub-cellular localization of tyra-2. A translational reporter of the entire tyra-2 genomic locus (ptyra-2::tyra-2::GFP) was injected into tyra-2 lof animals at 1 ng/µL, revealing expression of the receptor in both soma and sensory cilia. (60x magnification, scale bar denotes 20 µm). f Expression of the translational reporter restores wild type behavior in a tyra-2 lof background, n ≥ 5 trials. Data presented as mean ± S.E.M; *p < 0.05, **p < 0.01, ***p < 0.001. One factor ANOVA with Sidak’s multiple comparison posttest