Fig. 3.

a Cytotoxicity and b cellular uptake of polycation/miRNA complexes at different N/P ratios in smooth mouse cells (SMCs). Cellular internalization of polycation/miRNA complexes at the N/P ratio of 10 in SMCs: c Confocal fluorescence images, d average intensity measured by MD, e relative expression levels of miR-145 and f related KLF4 protein expressions determined by western blot. (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, see “Statistical analysis” in “Methods”; n ≥ 3 independent experiments; error bars represent standard deviation; Source data of a, b, d–f are provided as a Source Data file)