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. 2019 Jul 18;10(8):551. doi: 10.1038/s41419-019-1783-y

Fig. 3. FBXO30 promotes BMP pathway activity.

Fig. 3

a HEK293 cells were transfected with siRNA against RARγ as indicated. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before BRE-luciferase activity was measured. Data are mean ± s.d. (n = 3). b HEK293 cells were transfected with the FBXO30 together RARγ as indicated. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before BRE-luciferase activity was measured. Data are mean ± s.d. (n = 3). c HEK293 cells were transfected with the siRNA-FBXO30 and RARγ as indicated. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before BRE-luciferase activity was measured. Data are mean ± s.d. (n = 3). d HEK293 cells were transfected with the increasing amounts of FBXO30. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before BRE-luciferase activity was measured. Data are mean ± s.d. (n = 3). e HEK293 cells were transfected with the FBXO30 WT or deletion mutants as indicated. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before BRE-luciferase activity was measured. Data are mean ± s.d. (n = 3). f, g Overexpression of FBXO30 or siRNA-FBXO30 transfected in HEK293 cells as indicated. Thirty-six hours after transfection, cells were treated with BMP-2 (100 ng/ml) for 12 h before cell lysates harvest. Western blot was performed to examine the pSmad1/5 and total Smad1/5 levels in whole-cell lysates. Aliquots of total lysates were immunoblotted to indicate antibody. Numbers at the bottom were generated by quantification (Image J) of the pSmad1/5 signal normalized to the Smad1/5 signal. h siRNA-FBXO30 or siRNA-control was transfected into HEK293 cells. Direct visualization or indirect immunofluorescence was performed. Scale bars, 22 μm