Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 18;10(8):551. doi: 10.1038/s41419-019-1783-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a HEK293 cells were transfected overexpression of FBXO30. Thirty-six hours after transfection, BRE-luciferase activity was measured after treatments with BMP-2 (100 ng/ml) and/or RA (1 μM) for 12 h. Data are mean ± s.d. (n = 3). b HEK293 cells were transfected with the siRNA-FBXO30. Thirty-six hours after transfection, BRE-luciferase activity was measured after treatments with BMP-2 (100 ng/ml) and/or RA (1 μM) for 12 h. Data are mean ± s.d. (n = 3). c, d Overexpression of FBXO30 or siRNA-FBXO30 transfected in HEK293 cells as indicated. Thirty-six hours after transfection, cells were stimulated with BMP-2 and/or RA for 12 h before harvesting. Western blot was performed to examine the pSmad1/5 and total Smad1/5 levels in whole-cell lysates. Aliquots of total lysates were immunoblotted to indicate antibody. Numbers at the bottom were generated by quantification (Image J) of the pSmad1/5 signal normalized to the Smad1/5 signal. e siRNA-FBXO30 or siRNA-normal transfected in HEK293 cells as indicated. Thirty-six hours after transfection, cells were stimulated with BMP-2 and/or RA for 12 h before harvesting. Direct visualization or indirect immunofluorescence was performed. Scale bars, 22 μm