Fig. 7. FBXO30 downregulated with decreased BMP related gene expression in high-retinoid NTDs.

a Retinoid and Vitamin A contents in brain tissue of normal fetus and fetus with NTDs. b The mRNA expression of FBXO30 and RARγ in the brain of NTD fetuses, determined by Nano String. Data are mean ± s.d. (n = 10), *p < 0.05, by Student’s t test. c Detection of FBXO30 in brain tissues from human anencephaly and normal cases by western blot. Total GAPDH was used as a loading control. Data are mean ± s.d. (n = 3), *p < 0.05, by Student’s t test. d The mRNA expression of BMP related Genes in the brain of NTD fetuses, determined by Nano String. Data are mean ± s.d. (n = 10), *p < 0.05, by Student’s t test. e Pearson’s correlation analysis between FBXO30 expression and ID2, chordin expression