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. 2019 Jul 18;10(8):549. doi: 10.1038/s41419-019-1787-7

Fig. 4. DOX treatment suppressed TCTP expression both in vivo and in vitro.

Fig. 4

a, b mRNA level (a) and protein expression (b) of TCTP in NRVMs treated with or without DOX (0.1 μM) for different times (n = 4). c C57BL/6 mice were treated intraperitoneally with DOX (20 mg/kg) or normal saline (CTRL). Protein expression of TCTP and GAPDH in hearts was analyzed after DOX treatment for 48 h (n = 7–8). d C57BL/6 mice were treated intraperitoneally with DOX (3 mg/kg) or normal saline. DOX was administered three times a week, with a total dose of 24 mg/kg. At 5 weeks after the first injection, protein expression of TCTP and GAPDH in hearts was analyzed (n = 4). e, f WT and p53 KO mice were treated intraperitoneally with DOX (20 mg/kg) or normal saline (CTRL). Protein expression of TCTP (e) (n = 11–12), Bnip3 (f) (n = 5~6) and GAPDH in hearts was analyzed after 48 h. *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA followed by Tukey’s test (a, b) or unpaired, two-tailed Student’s t-test (c, d, e <bottom> , f <bottom>) or two-way ANOVA followed by Bonferroni’s test (e <top> , f <top>)