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. 2019 Jul 18;10(8):549. doi: 10.1038/s41419-019-1787-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — H9C2 cells were transfected with pcDNA3.1-control vector or pcDNA3.1 mouse TCTP expression plasmid (mTCTP) for 24 h and then treated with DOX (0.1 μM) or vehicle for 48 h. a Protein expression of TCTP and GAPDH was analyzed by western blotting (n = 8). b Cell death was determined by calcein-AM/ethidium homodimer-1 staining (n = 4). Live and dead cells were distinguished by calcein-AM (green) and ethidium homodimer-1 (red) staining, respectively. Scale bar, 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way ANOVA followed by Bonferroni’s test