Fig. 7. DHA treatment resulted in cardiac dysfunction accompanied with reduced expression of TCTP in the heart.

WT and TCTP TG mice were treated intraperitoneally with DHA (30 mg/kg) or normal saline (CTRL) once a day for 4 weeks. a Protein expression levels of TCTP, Bnip3, and GAPDH in the heart were analyzed by western blotting (n = 5–7). b LVEF was evaluated by echocardiography (n = 10–11). c, d Max dP/dt and Min dP/dt were evaluated by cardiac catheterization (n = 9–11). e Cardiac apoptosis in the myocardium was assessed by TUNEL assay in heart sections from WT and TCTP TG mice given DHA or vehicle treatment. Nuclei were stained with DAPI (blue). Representative images are shown to the left. The white arrows indicate apoptotic cells in the sections (n = 5). Scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA followed by Tukey’s test