Fig. 5.

Local glutamate uncaging at spines triggers Rgs4 3′-UTR-dependent mRNA recruitment. a Representative GFP fluorescence of a hippocampal neuron cotransfected with the MS2+Rgs4 3′-UTR reporter and tdMCP-GFP constructs before (left panel) and after (middle, right panels) local glutamate uncaging. Black arrowhead denotes the uncaging spot at dendritic spine. White arrowheads indicate GFP positive MS2+Rgs4 reporter mRNA granules. Scale bar 2 µm. b Dot plot displaying the change in RNA granule number 40–45 min after uncaging compared to before uncaging within 5 µm of the stimulated spine. Data represent mean ± standard deviation (individual neurons shown as gray dots). Asterisks represent p-values obtained by Student’s t test (***p < 0.001). Data were obtained from six dendrites for MS2 only (five neurons of four biological replicates) and five dendrites for MS2+Rgs4 reporter mRNAs (five neurons of five biological replicates), respectively