Fig. 6.

Stau2 regulates Rgs4 3′-UTR-dependent transport. a Representative dual-color kymograph showing MS2+Rgs4 3′-UTR reporter mRNA (green) and tagRFPt-tagged Stau2 (magenta) from a dendrite of a rat hippocampal neuron. First and last frames are shown at top and bottom. Arrowheads indicate an MS2+Rgs4 reporter and Stau2 positive RNA granule undergoing cotransport. Scale bar 5 µm. Dot plots (b, d) and histograms (c, e) displaying percentage of anterograde moving MS2 only or MS2+Rgs4 3′-UTR reporter mRNA granules in shNTC and shStau2 transduced hippocampal neurons. NTC non-targeting control. Data represent mean ± standard deviation of three independent experiments (individual experiments shown as gray dots). Asterisks represent p-values obtained by Student’s t test (*p < 0.05). Data were obtained from 40-µm dendritic segments at a minimal distance of 20 µm from the cell body. Total number of dendrites (n_d) and tracks (n_t) analyzed per condition are indicated. Only displacements ≥1.5 µm were considered for analysis