Fig. 3. DN Grb2 attenuates the DNA homologous recombination repair in response to oxidative stress.

a Schematic representation of the WT and mutant DN Grb2. b HeLa cells were transfected transiently with the GFP-vector, WT Grb2, or -DN Grb2 plasmids for 36 h, and then treated with 0.5 mM H₂O₂ for 2 h. The number of cells containing micronuclei was observed under microscope and shown in graph. c HeLa cells were transfected with the control (Mock) or Grb2 siRNA (siGrb2) for 48 h, and treated with 0.5 mM H₂O₂ for 2 h. Cell lysates were subjected to immunoblotting with the indicated antibodies. d, e HeLa cells were transfected transiently with the GFP-vector, WT Grb2 or DN Grb2 plasmids for 36 h, and treated with 0.5 mM H₂O₂ for 2 h. Cell lysates were analyzed by immunoblotting with the indicated antibody. For histogram results, the data were presented as mean ± S.D. and analyzed by T-test. *P < 0.05 vs. control; **P < 0.01 vs. control. Similar experiments were repeated at least three times