Fig. 4. PTEN binds to Rad51.

a–c HeLa cells were transfected with indicated siRNAs for 48 h, and treated with 0.5 mM H₂O₂ for 2 h. Cell lysates were subjected to immunoblotting with the indicated antibodies. d Schematic representation of the WT and mutant PTEN. e HeLa cells were transiently transfected with the WT and mutated PTEN for 36 h, stained with DAPI and PTEN antibodies, and observed with immunofluorescence microscope. f HeLa cells were transfected with the plasmids expressing PTEN for 36 h. Equal amounts of cell lysates prepared from transfection samples were immunoprecipitated with the antibody to Rad51. Immunoprecipitates and lysate inputs were then blotted with indicated antibodies. g HeLa cells were transfected with the control (Mock) or Grb2 siRNA (siGrb2) for 48 h, equal amounts of cell lysates were immunoprecipitated with the antibody of PTEN or IgG. Immunoprecipitates were then blotted for PTEN or Rad51. Similar experiments were repeated at least three times. L-Exp long time exposure