Fig. 9.

PKB/Akt promotes HDAC4 nuclear import in muscle cells. a, b Fluorescent pictures of C2C12 myotubes co-electroporated with a wild-type form (WT) of HDAC4 tagged with GFP or with a nuclear mutant (HDAC4-3SA), together with an inhibited (inh), a wild-type (wt) or a constitutively active (ca) form of Akt1 tagged with HA. Scale bar, 50 µm. The quantification in (b) gives the proportion of myotubes with only cytoplasmic HDAC4 localization; data are mean ± s.e.m.; total myotubes counted = 406 (−), 322 (inh), 356 (wt), 205 (ca); one-way ANOVA with Tukey’s post-hoc test, *p < 0.05, **p < 0.01. c Scheme illustrating the role of mTORC1 and PKB/Akt in the muscle response to denervation. Source Data are provided in the Source Data File