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. 2019 Jul 18;9:10416. doi: 10.1038/s41598-019-46796-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo monitoring of the concentration of Zn in three points of the stem and in the petiole of Phaseolus vulgaris whose roots were immersed in 100 and 1000 mg Zn L⁻¹; (a) the location in which the measurements were performed, and the uptake of Zn in plants exposed to (b) ZnSO_4(aq), (c) 40 nm ZnO, (d) 300 nm ZnO. The concentration of Zn decreases from root to shoot, except for 1000 mg Zn L⁻¹ ZnSO_4(aq). The Zn counts at the time corresponding to zero hour were recorded immediately before the plants were exposed to the treatments.