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. 2019 Jul 18;9:10416. doi: 10.1038/s41598-019-46796-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ZnSO_4(aq) and ZnO NPs on expression profiles of metal transporters. Relative transcriptional levels of PvZIF1, PvNRAMP3 and -4, PvMTP1, -8, PvIRT3 and PvHMA2, in shoots and roots of P. vulgaris seedlings submitted to 1000 mg/L ZnSO_4(aq), 40 nm and 300 nm ZnO NP for 1, 24 and 48 h. Values correspond to the averaged expression data shown as fold change between treated and control plants across three biological repetitions containing two seedlings each. Error bars denote standard errors (SE). *Indicates significant differences compared to the mock treatment by Student’s t-test with equal variances.