Fig. 3. SMC-specific HIF1α deficiency abolishes Ang II-induced M1 macrophage infiltration and vascular inflammation.

Hif1a^fl/fl and Hif1a^ΔSMC mice were infused with saline or 1000 ng/kg/min Ang II for 28 days. a Il1b, Il6, Tnfa, Mcp1 mRNAs in aortas after saline or angiotensin II infusion for 28 days were measured by qPCR. *P < 0.05, **P < 0.01, n = 6 per group. b Immunofluorescence staining of representative cross-sections of mice aortas for the F4/80-positive (green) cells and quantification (c) *P < 0.05, n = 6, statistical significance was determined by the unpaired t-test. Flow cytometry analysis was performed for the aortas (d, j) and CD45⁺ cells (e), CD45⁺CD11b⁺F4/80⁺ macrophages (f), CD45⁺CD11b⁺F4/80⁺CD206⁻ M1 macrophages (g), CD45⁺CD11b⁺F4/80⁺CD206⁺ M2 macrophages (h), CD45⁺CD11b⁺LY6G⁺ neutrophils (i), CD45⁺CD3⁺ T cells (k), CD45⁺CD3⁺CD4⁺ T cells (l),CD45⁺CD3⁺CD8⁺ T cells (m), and CD45⁺CD3⁺NK11⁺ NKT cells (n) were quantified, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, n = 6 per group. Statistical significance was determined by one-way ANOVA test followed by the unpaired t-test