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. 2019 Jul 19;39(7):BSR20190280. doi: 10.1042/BSR20190280

Figure 1. LncRNA PFAR acts as molecular sponge of miR-15a.

Figure 1

(A) qRT-PCR analysis of the expression of miR-15a in the lungs of mice after injection of AAV5-sh-PFAR. qRT-PCR analysis of the expression of miR-15a in lung fibroblasts after PFAR inhibition (B) or overexpression (C). (D) Predicted binding sites of PFAR and miR-15a. PFAR-Mut, mutated binding site. (E) Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of PFAR containing WT or mutated miR-15a-binding site. (F) Lung fibroblasts were co-transfected with the miR-15a sensor and PFAR/sh-PFAR and its corresponding scrambled form, and luciferase activity was detected. (G) Lung fibroblasts were co-transfected with the miR-15a sensor, miR-15a and PFAR and its corresponding scrambled form, and luciferase activity was detected. All data are presented as mean ± SEM. n=6 independent experiments. *P<0.05; NS, not significant.