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. 2019 Jul 19;39(7):BSR20190668. doi: 10.1042/BSR20190668

Figure 5. MiR-205 regulates glioma glucose uptake, migration and invasion by targeting E2F3.

Figure 5

(A) StarBase v3.0 was used to predict the binding site of miR-205 within the 3′-UTR of E2F3. (B) Expression of E2F3 in clinic glioma tissues. (C,D) Expression of E2F3 in glioma cell lines and NHAs (qRT-PCR and Western blot). (E-F) Luciferase reporter assay showed that miR-205 decreased the luciferase activity of E2F3 luciferase reporters obviously. (G–I) Expression of E2F3 in cells transfected with NC, miR-205 or miR-205 together with E2F3 (qRT-PCR, Western blot and immunofluorescence). (J,K) Glucose uptake assay was used to measure the glucose uptake of cells transfected with NC, miR-205 or miR-205 together with E2F3. (L,M) Migration assay was performed to explore the migration capacity of cells transfected with transfected with NC, miR-205 or miR-205 together with E2F3. (N,O) Transwell assay was applied to explore the invasion ability of cells transfected with NC, miR-205 or miR-205 together with E2F3. *P<0.05, **P<0.01. GAPDH used as control.