Fig. 3.

Callus induction from the mature seeds after 10 days and the different hormone treatments of rgb1 mutants, RGB1 heterozygous mutants, and WT.

(a) Mature seeds of the RGB1 heterozygous mutant lines and WT cultured on callus preinduction media (N6D2). (a1) WT control, (a2) SG948–6 (Aa), (a3) SG948–8 (Aa), (a4) SG948–13 (Aa) (Aa indicates heterozygous RGB1/rgb1 mutants). (b) Calli induced from the rgb1–1 and rgb1–2 mutants compared with WT. (c) Statistical analysis of shoot lengths of the WT and the rgb1 mutants shown in (b). The data are the means ± SDs (n = 10; **p < 0.01). (d) Comparison of the morphology of calli from the WT and the rgb1 mutants. (e) Median longitudinally dissected embryos of WT and rgb1 mutants (CO, coleoptile; RA, radicle). (f) Phenotypic observations of 1-week-old seedlings of the rgb1 mutants and WT under 1 μM BRs, 3 μM BRs, 1 μM IAA, 5 μM IAA, and 1 μM GA. Scale bar, 1 cm