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. 2019 Jul 10;26:101273. doi: 10.1016/j.redox.2019.101273

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 10 — CYR5099 does not alter Ca²⁺mobilization in activated human neutrophils. Fluo-3-labeled human neutrophils were incubated with DMSO or CYR5099 (10 μM) for 5 min, and then activated by DMSO, (A) fMLF (0.1 μM), (B) f-MMYALF (0.3 μM), (C) MMK1 (0.3 μM), (D) m-3M3FBS (15 μM), (E) LTB₄ (0.1 μM), (F) IL-8 (100 ng/ml), or (G) PAF (1 μM). Ca²⁺ mobilization was determined in real-time using a spectrofluorometer. Representative traces of Ca²⁺ mobilization are shown (n = 3 or 4).