Fig. 12.

CYR5099 inhibits human neutrophil CD11b expression and adhesion. Human neutrophils were incubated with DMSO or CYR5099 (5 and 10 μM) for 5 min, and then activated by (A) fMLF (0.1 μM)/CB (0.5 μg/ml) or (B) PMA (10 nM) for an additional 5 min. The fluorescent intensity was determined by FACS using FITC-labeled anti-CD11b antibody. Fluorescence intensities of CD11b are represented as mean ± S.E.M. (n = 6). (C) Hoechst 33342-labelled human neutrophils were incubated with DMSO or CYR5099 (5 and 10 μM), and activated by fMLF (0.1 μM)/CB (0.5 μg/ml). Drug-treated neutrophils were then co-incubated for 15 min with LPS-preincubated bEnd.3. Adherent neutrophils were detected by fluorescent microscopy. Bar = 100 μm. Representative images are shown. (D) Data from (C) are expressed as mean ± S.E.M. (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with fMLF or PMA alone.