Fig. 4.

CYR5099 does not affect elastase release in activated human neutrophils. Human neutrophils were incubated with DMSO or CYR5099 (1–10 μM) for 5 min, and then activated by (A) fMLF (0.1 μM)/CB (0.5 μg/ml), (B) f-MMYALF (0.3 μM)/CB (0.5 μg/ml), (C) MMK1 (0.3 μM)/CB (0.5 μg/ml), (D) m-3M3FBS (15 μM)/CB (0.5 μg/ml), (E) LTB₄ (0.1 μM)/CB (0.5 μg/ml), (F) IL-8 (100 ng/ml)/CB (2 μg/ml), or (G) PAF (1 μM)/CB (0.5 μg/ml). Elastase release was measured spectrophotometrically at 450 nm. All data are expressed as mean ± S.E.M. (n = 4 or 6).