Fig. 5.

CYR5099 does not inhibit p47^phoxtranslocation in human neutrophils. Human neutrophils were incubated with DMSO or CYR5099 (10 μM) for 5 min, and then activated PMA (10 nM). Cells were fixed with 4% paraformaldehyde and stained with anti-p47^phox antibody, DiI, and Hochst 33342. p47^phox, DiI, and Hoechst 33342 staining are shown in green, red, and blue, respectively. Co-distribution (orange) was determined by merging the green and red images. Scale bar: 2 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)