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. 2019 Jul 12;10:909. doi: 10.3389/fpls.2019.00909

FIGURE 3.

FIGURE 3

Effect of exogenous Fe supply to shoots on the WCS417-induced Fe deficiency response in roots. (A) qRT-PCR analysis of FER1 gene expression in roots and shoots of 19-day-old Col-0 plants (B), 7 days after exogenous application of indicated FeNaEDTA concentrations to the shoot. (C) Shoot FW of WCS417-inoculated and non-inoculated plants, 7 days after shoot Fe supply. (D,E) qRT-PCR analysis of MYB72, IRT1, or FER1 gene expression in roots or shoots, 2 days after shoot Fe supply. Shoots of 12-day-old Col-0 plants growing on Fe-sufficient medium were supplemented with Fe by applying 0.2 μl of a solution with indicated concentrations of FeNaEDTA onto two leaves per plant. Root inoculations with WCS417 (OD600 = 0.01) were performed just prior to supply of Fe to the shoots. Gene expression levels were normalized to that of the constitutively expressed reference gene PP2AA3 (At1g13320). Plotted are fold changes in gene expression levels relative to the average of non-inoculated, mock-treated plants. Error bars represent standard errors of the mean. Per timepoint, different letters indicate statistically significant differences between treatments (one-way ANOVA followed by Tukey’s test; P < 0.05; n = 5-6). Scale bars in panel (B) represent 1 cm.